Routine:
BA, CNA, MAC, THIO, and gram stain

STOOL:
HE, XLD, CNA, MAC

SPUTUM:
BA, CNA, MAC, CHOC

CSF:
BA, CNA, MAC, CHOC, THIO, g.s.

URINE: colony count
BA, CNA, MAC, wet mount/ NOOOO g.s.

UROGENITAL DISCHARGE:
BA, THAYER-MARTIN, CHOC

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

GRAM POSITIVE COCCI
|
V
catalase
+-------------------------------------------------- =
Staph Strep
| |
V V
coagulase hemolysis
+---------------------= a---------------------b---------------none
S. aureus Staph coag= | | |
V | |
optochin | |
S+------------R= | |
S. pneumo | | |
| | |
V | |
Bile | |
Esculin | |
+------------= | |
6.5% NaCl Viridans | |
+--------------= group | |
Enterococcus Group | |
spp. D V |
Bacitracin |
S+---------------R= |
S. pyogenes | |
Strep Group A V |
CAMP |
+----------------= |
S. agalactiae | |
Strep Group B | |
V |
Bile |
Esculin |
+--------------= |
6.5% NaCl Viridans |
+------------------= Group |
Enterococcus Group |
spp. D V
Bile
Esculin
+-----------------=
6.5% NaCl Viridans
+---------------= Group
Enterococcus Group
spp. D
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

GRAM NEGATIVE BACILLI
|
|
V
Oxidase
+---------------------=
Pseudomonas |
[NLF] |
V
Indole
+----------------=
E. coli Klebsiella
pneumoniae

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
GRAM POSITIVE BACILLUS
|
|
|

chapter 4: B

4Safety levels:
1) specimens not known to be potentially hazardous
2) all common agents of infections, including HIV&HBV
3) certain viruses and easily transmitted bacteria.
4) only certain viruses of arbovirus, arenavirus, or filovirus groups. None are common in the US

Micro role:
a) handle
b) test
c) identify

Enterobacteriaceae

-motile with the exception of Yersenia, Shigella, and Klebsiella
-ferment glucose (referred to as "fermenters")
-oxidase negative
-gram negative
*key terms* {obligate aerobes, aerotolerant anaerobes, obligate anaerobes,
facultative anaerobes, and capnophilic microaerophilic} *key terms*
-enterics (found mostly in the intestines)
-reduce nitrate to nitrite
-MAC and EMB plates
-GI specimens (stools) call for HE, XLD and GN broth
-large, shiny, moist, gray/grayish white. may or may not be hemolytic, translucent and
slightly convex, some mucoid
-utilize: carbohydrates, citrate, urease, indole.
-produce hydrogen sulfide (H2S), metabolize tryptophan (amino acid)
-IMVIC:
indole, methyl red, voges proskauer, citrate

Rambles of Sections and G-stain

-microbiology
-bacteriology
-mycology
-parasitology
-virology

prokaryotic-lack nuclear membrane and organelles, function takes place in cytoplasm.
g=:red
g+:blue

cocci-round
coccobacilli-oval
bacilli-rod
fusiform-pointed end

arrangement:
-single
-pairs (diplo)
-tetrads
-chains
-clusters
-pallisades

components:
outer membrane, cell wall (murein layer), periplasma, cytoplasma, pilus

cystosol, polysomes, inclusions, nucleoids

Ch. 6

maintain viability of organisms with none-minimum contamination.

Agars:
CHOC-
CNA-
MAC-
TSA-

Gram Stain:
1) crystal violet-1min
2) iodine-1min
3) Alcohol acetone-20sec
4) Safranin-30sec